PET3a 大肠杆菌原核表达质粒
pet3a载体基本信息
| emd biosciences(novagen) |
| pet3a, pet 3a |
| 大肠杆菌蛋白表达 |
| 高 |
| 多克隆位点，限制性内切酶 |
| 4640bp |
| t7 |
| 5'-taatacgactcactataggg-3' |
| n-t7 |
| ampicillin |
|
same as pet9 but ampr; a,b,c,d vary by mcs.
|
| 69418-3, 69419-3, 69420-3, 69421-3 |
| 瞬时表达 transient |
| 组成型 constitutive |
| 非病毒 |
locus pet-3a(+) 4640 bp dna syn
definition pet-3a
comment this file is created by vector nti
http://www.biofeng.com/
comment origdb|genbank
comment vntauthorname|biofeng.com|
comment vntoauthorad1|novagen inc.|
organism other sequences; artificial sequences; vectors.
features location/qualifiers
source 1..4640
/organism="pet-3a"
/mol_type="other dna"
gene 86..1553
/label="tet (w/ gaps)"
/gene="tet (w/ gaps)"
terminator complement(379..507)
/label="t7_terminator"
misc_feature 447..465
/label="t7_terminal_primer"
misc_feature complement(519..551)
/label="t7_leader"
misc_feature complement(534..552)
/label="xpress_fwd_primer"
misc_feature complement(560..576)
/label="t7_transl_en_rbs"
promoter complement(613..631)
/label="t7_promoter"
misc_feature complement(715..734)
/label="pbrrevbam_primer"
cds 768..1553
/label="orf frame 3"
cds complement(900..1358)
/label="orf frame 1"
misc_feature 2192..2383
/label="rop"
misc_feature complement(2377..2399)
/label="pgex_3_primer"
gene complement(3572..4432)
/label="ampicillin"
/gene="ampicillin"
cds complement(3572..4432)
/label="orf frame 2"
promoter complement(4474..4502)
/label="ampr_promoter"
origin
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc
541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac
601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag
961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
4561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
4621 ggccctttcg tcttcaagaa
//
definition pet-3a
comment this file is created by vector nti
http://www.biofeng.com/
comment origdb|genbank
comment vntauthorname|biofeng.com|
comment vntoauthorad1|novagen inc.|
organism other sequences; artificial sequences; vectors.
features location/qualifiers
source 1..4640
/organism="pet-3a"
/mol_type="other dna"
gene 86..1553
/label="tet (w/ gaps)"
/gene="tet (w/ gaps)"
terminator complement(379..507)
/label="t7_terminator"
misc_feature 447..465
/label="t7_terminal_primer"
misc_feature complement(519..551)
/label="t7_leader"
misc_feature complement(534..552)
/label="xpress_fwd_primer"
misc_feature complement(560..576)
/label="t7_transl_en_rbs"
promoter complement(613..631)
/label="t7_promoter"
misc_feature complement(715..734)
/label="pbrrevbam_primer"
cds 768..1553
/label="orf frame 3"
cds complement(900..1358)
/label="orf frame 1"
misc_feature 2192..2383
/label="rop"
misc_feature complement(2377..2399)
/label="pgex_3_primer"
gene complement(3572..4432)
/label="ampicillin"
/gene="ampicillin"
cds complement(3572..4432)
/label="orf frame 2"
promoter complement(4474..4502)
/label="ampr_promoter"
origin
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc
541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac
601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag
961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
4561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
4621 ggccctttcg tcttcaagaa
//
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